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无土栽培模式下施用有机物质对番茄生长、果实品质和根际微生物环境的影响 (第2页) - 农业 - 无忧论文网

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无土栽培模式下施用有机物质对番茄生长、果实品质和根际微生物环境的影响

日期:2021年11月11日 编辑:ad201107111759308692 作者:无忧论文网 点击次数:841
论文价格:150元/篇 论文编号:lw202110251433438104 论文字数:59899 所属栏目:农业论文
论文地区:中国 论文语种:中文 论文用途:硕士毕业论文 Master Thesis
相关标签:农业论文
R 引物和ITS1F/ITS2R 引物进行 PCR 扩增;将同一样本的 PCR 产物混合后使用 2%琼脂糖凝胶回收 PCR 产物,利用 AxyPrep DNA Gel Extraction Kit(Axygen Biosciences, Union City,  CA,  USA)进行回收产物纯化,2%琼脂糖凝胶电泳检测,并用 Quantus™ Fluoromete(rPromega, USA)对回收产物进行检测定量。使用 NEXTFLEX Rapid DNA-Seq Kit 进行建库。最后利用 Illumina 公司的 Miseq PE300/NovaSeq PE250 平台进行测序。

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6  讨论与结论

本论文通过两组试验,对无土栽培下施用有机物质对番茄生长和果实品质的影响进行了研究。叶面肥中施用的有机物质为沼液提取物和宛氏拟青霉菌,水溶肥中施用的有机物物质为沼液水溶肥和有机碳肥,二者都以常用的化学叶面肥和水溶肥作为对照,进行了全面的研究与分析。植株生长量在植物生长过程中起着重要作用,是植物获取能量、合成有机物质、增加产量和提升品质的重要保障。通过对叶面肥试验中番茄生长量和叶绿素含量的测定发现,在无土栽培下施用含有有机物质的叶面肥在促进番茄植株鲜质量、根冠比和含水率方面均高于化学叶面肥,其中鲜质量比化学叶面肥提高了 17.37%~43.06%,根冠比比化学叶面肥提高了 6.96%~91.65%,含水率比化学叶面肥提高了 2.41%~4.73%,干质量和壮苗指数也均高于化学叶面肥,可见在叶面肥中施用有机物质有利于促进番茄植株生长量。通过对水溶肥试验中番茄生长量、光合作用和根系活力的测定发现,施用有机物质对番茄生长量并无显著效果。产量通常是衡量作物经济效益的一个值指标,高产量往往能带来更好的经济效益。通过对叶面肥试验中番茄产量和货架期的测定发现,在无土栽培下施用含有有机物质的叶面肥在促进番茄开花数和坐果数方面均高于化学叶面肥,其中开花数比化学叶面肥提高了 5.01%~30.30%,坐果数比化学叶面肥提高了 2.06%~68.86%,坐果率和单株产量也普遍高于化学叶面肥。通过对水溶肥试验中番茄产量和货架期的测定发现,施用有机物质对番茄产量和货架期并无显著效果。在果实品质方面,两个试验均显示施用有机物质对果实品质无显著效果。综上所述,无土栽培下,在叶面肥中施用有机物质有利于促进番茄植株生长,增加番茄产量,但在水溶肥中施用有机物质对番茄的生长、产量和果实品质均无显著效果,这可能是有机物质在无土栽培基质中分解速率较慢,无法在短期试验内对番茄生长产生明显的促进作用,具体原因还有待进一步研究。

参考文献(略)